Effect of uric acid on vitamin C and E in induced hyperuricemic model

To measure the level of uric acids and find out the effect of uric acid on vit C and E in induced hyperuricemic model. Comparative study. This study was conducted at Baqai Medical University, Karachi from June 2010 to January 2011. Forty male albino rats with an average weight of 180 +/- 2 g were selected. The rats were grouped. The animals were fed on standards diet and given tap water ad libitum untril treatment. The protocols for experiment was according to institute of laboratory animal resources on life sciences, US National research council, 1996 and institutional animal ethical committee [IAEC] of baqai medical university, Karachi. Albino rats were divided into four groups. Group [A] 10 - control given only standard diet, group B[10] fed on 60% fructose with standard diet, group C[10] fed on fructose, standard diet and intraperitonially oxonic acid 250 mg/kg and group D[10] only on injection intraperotonially oxonic acid 250mg/kg. at the end of study 10 ml of blood was drawn from heart of rats. Then blood was estimated for vitamin C,E and uric acids done by kit methods randox-manual /Rx monza UA230/UA233. The concentration of vitamin C and E were significantly lowered as compared to uric acid concentration in the group B, C and D. Decrease level of vit C and E increase the level uric acids were observed. Therefore, it may be suggested that increase intake of vitamin C may be helpful in lowering uric acid concentration

Effects of oral isotretinoin subsequent to diamond dermabrasion of post acne scaring

Background: Acne vulgaris is treated by topical and or systemic antibiotics, vitamins and some keratolytics. Micro dermabrasion, a modified technique, useful for scar removal is more natural, gentler and less invasive tool for doing exfoliation

Objective: To observe the comparative effects of diamond dermabrasion on post acne/inflammatory scaring with or without oral Isotretinoin and its probable side effects

Patients and Methods: A total of 232 patients of post acne/inflammatory scaring were randomly enrolled. In this experimental study out of which 159 females were divided into two groups, Fl of 80 and F2 of 79 females. The F1 was prescribed on Isotretinoin 20 mg/day for 45 successive days after diamond dermabrasion performed by NOVA NV60. F2 received no further treatment after the procedure. The males were divided into Ml and M2, of 37 and 36 in each group respectively and were given isotretinoin 20mg/day in Ml group and no treatment in M2 group

Results: In female groups, 82.55% experienced non-inflammatory lesions, erythema in 80%, desquamation in 21.25%, mild to moderate inflammatory lesions in 17.5%, dryness in 88.75%, pruritus in 32.50% and stinging /burning in 13.75% was observed in Group F1. In group F2 these percentages were 74.68%, 77.2%, 17.72%, 25%, 79.74%, 43.03%, and 24.05% of the same parameters, respectively. F1were graded for progress, 02 females were of grade 0, 13 women obtained grade 1, 41 patients of Grade 2 and 24 females were of Grade 3. The progress grades for F2 were, Grade O had 11 females, Grade 1 had 27, Grade 2 had 38 while Grade 3 had only 03 females. In male group Ml, 78.37% experienced non-inflammatory lesions, erythema in 83.78%, 29.72% had desquamation, 21% had mild to moderate inflammatory lesions, dryness in 89.18%, pruritus in 78.37% and stinging/burning in 16.20% in Group Ml. In group M2 these percentage s were 83.33%, 94.44%, 36.11, 25%, 83.33%, 77% and 27.77% of the same parameters, respectively. Progress grades for M1 were; Grade O for 04, Grade 1 for 10, Grade 2 for 16 and Grade 3 for 07. For M2 the same grading system was followed and Grade O had 02, Grade 1 for 19, Grade 2 were 11 and Grade 3 was 04

Conclusion: The suggestion of prescription oflsotretinoin following Diamond Dermabrasion is due to the powerful epithelial generation by the vitamin A analogue. This effect provides good and early healing of the abraded skin. However further studies are suggested with larger sample size